【学术报告·高等讲堂】Daniel Razansky：Super-resolution deep tissue imaging of microcirculation with labeled particles and cells
Microvessels smaller than 100 microns are the principal sites of exchange for oxygen, nutrients, and waste, yet their minute scale has long impeded detailed observation. Recently, localization-based microangiography methods leveraging fluorescence, ultrasound, and hybrid optoacoustic contrast have emerged, breaking traditional resolution-depth trade-offs and offering unprecedented noninvasive quantification of cerebrovascular anatomy and blood flow down to the capillary scale. Inspired by single-molecule localization microscopy, these approaches exploit flowing exogenous particles or labeled cells as sparse emitters, overcoming diffraction- and diffusion-related resolution limits in deep living tissues while enabling simultaneous extraction of blood flow velocity and direction.
